18 April 2019 |       Share
 
 
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CASE STUDIES
Myelotoxicity
Skin Wound Healing
Skin Toxicity Study
Endocrine Disruptors
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Lipid Metabolism
Carcinogenicity
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Carcinogenicity


Quantification of the drug effects is related to the biological response of the organism and can be measured in time, in the same animal.
Using repTOPTMmitoIRE mouse we can detect hyperproliferative events linked to early cancerogenesis in the living animal, by non-invasive and very sensitive imaging techniques.

We therefore induced formation of skin papillomas by local administration of 7,12-dimethylbenz(a)anthracene and phorbol ester 12-O-tetradecanoylphorbol-13 acetate (DMBA-TPA) to the skin of MITO-Luc mice. DMBA and TPA were put directly onto shaved ventral skin, with DMBA applied once-weekly and TPA applied twice-weekly. We detected luminescence at the treated sites directly after the first DMBA application and during the DMBA-TPA treatment period prior to tumor occurrence, which took place between 12 and 14 weeks after treatment.



(a) BLI of a representative MITO-Luc mouse after 2, 4, 6, and 8 wk of DMBA-TPA treatments on ventral skin. (b) Quantification of emitted light from MITO-Luc mouse skin papillomas. The photon emission at the treated sites was detected very soon prior to tumor occurrence, which took place between 12 and 14 wks after treatment. In fact, hyperproliferation represents an early occurring pre-neoplastic event.