20 Febraury 2019 |       Share
 
 
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PPRE-Luc mouse model
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A novel insights on our repTOP ERE-Luc mouse model
In a recent publication: “Estrogen receptor...
Join TOP srl at the 12th FELASA and 12th SECAL joint congress entitled: ‘Animal Research: Better Science from Fewer Animals’ (10-13 June 2013 Barcelona)
This year at the FELASA meeting, TOP srl will...
Join TOP srl at the American Association for Cancer Research (AACR) Annual Meeting 2013 (April 6-10 2013 Washington DC)
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Introducing the repTOP™ ERE-Luc model

“ERE” is the Estrogen receptor  Responsive Element

Follow the webinar on Charles River web site.

Thanks to our propietary technology repTOP™ERE-Luc transgenic reporter mice, express the luciferase gene in every tissue where Estrogen Receptor alpha or beta is activating transcription. The expression of luciferase is tightly controlled by an artificial promoter responsive to the activity of ER alpha-beta.
 


   SATURN Validation results:
Specificity
reporter specifically regulated by Estrogen Receptor (ER) ligands.
Accuracy: quantitative bioluminescence readout upon stimulation with 0.5-250 μg/Kg 17-β-estradiol. Activation tested using several endogenous, as well as artificial, agonists (xenoestrogens, phytoestrogens).
Turn-over: luciferase reporter half-life is 2.6 hours in all tissues.
Ubiquity: ER-regulated expression has been detected in all tissues known to express ERs (more than 30 tissues examined).
Reproducibility: typical inter-individual variability in acute treatments in the liver with 17-β-estradiol (SEM: 15% in vivo; 7% ex vivo).
Non-invasive: suitable for in vivo optical imaging.

The repTOP™ERE-Luc mouse enabled the discovery that, during the estrous cycle of female animals, estrogen receptor activity does not only change in intensity due to different levels of circulating hormones, but is also varying across reproductive and non-reproductive organs, probably due to other factors interacting with the receptors in each different tissue (see Ciana P et al. Nat. Med, 2003). These observations were performed in untreated anesthetized mice, producing reliable signals just due to the physiological levels of circulating hormones. These findings demonstrate the sensitivity of the system, that, together with specificity and ubiquitous response, makes the repTOP™ERE-Luc model the ideal tool for studying in vivo pharmacological treatments interfering with the estrogen system.


rep
TOP™ERE-Luc
 In Vivo Giude on Charles River web site 
repTOP™ERE-Luc technical sheet on Charles River web site

 

References

Application areas:

·         Reproductive dysfunctions

·         Menopause and related diseases

·         Hormone-related neurological disorders (depression and neurodegeneration)

·         Inflammation

·         Cancer

·         Toxicological analysis of food and environment

 

Upon request, TOP srl is able to offer also repTOP™ERE-Luc cells and vectors.

For inquiries, please contact business.development@top-mice.com