11 December 2017 |       Share
 
 
ABOUT US
Nice to meet you!
   
TECHNOLOGY
Our edge for you
   
PRODUCTS
The main products
   
SERVICES
We can help you!
   
CASE STUDIES
Model Applications
   
WEBINAR
Let's see how!
   
CONTACT
Get in touch!
 

repTOP™ mice and nutraceuticals

Besides its use for pharmaceutical research, repTOP™ technology increases productivity in other fields. With reporter mice it is possible to detect biologic effects not only of purified compounds, but also of complex mixtures like food and natural products.


Increased consumers’ awareness and care for wellness, together with progress in understanding and manipulating principles and substances are stimulating a gradual fading of borders between the food and pharma businesses. Integrating conventional and non-conventional medicine is an increasing trend. Both in the case of food extracts or in functional food design, repTOP™ mice can provide useful proof-of-principle and indicate ways for optimization.


We report here an example of the use of the repTOP™ ERE-Luc mouse for the characterization of the estrogenic effects of soymilk.

 


Isoflavones are a class of plant-derived compounds mimicking estrogen activity in mammals. Genistein and daidzein are two of such phytoestrogens contained in soybeans. Efficacy against several diseases of soy-based diets and derivative nutraceutical products is still matter of debate, as well as their safety.

We applied repTOP™ ERE-Luc mice to the study of estrogen system modulation due to genistein and in general soy-based diet. After feeding mice with soy milk for one day, estrogen receptor activity was increased of about three folds above control. This was somehow expected based on data about pure genistein, but anyway confirmed that also the natural occurring compound, in the context of a dietary mixture, was biologically active. Surprisingly we observed that soy milk feeding over prolonged times is much stronger in inducing and sustaining estrogen receptor activity than the administration of the purified genistein or of a mixture of genistein and daidzein, the two main phytoestrogens present in soy milk.



REFERENCES

OTHER APPLICATIONS

repTOP™ mice can be applied also to the study of

* Drug discovery
* Toxicology