20 Febraury 2019 |       Share
 
 
ABOUT US
Nice to meet you!
   
TECHNOLOGY
Our edge for you
   
PRODUCTS
The main products
   
SERVICES
We can help you!
   
CASE STUDIES
Model Applications
   
WEBINAR
Let's see how!
   
CONTACT
Get in touch!
 
TECHNOLOGY
Advantages & flexibility
Proprietary Technology
Validation
Applications
References
RESTRICTED AREA
Username
Password
Recover Password

NEWS

PPRE-Luc mouse model
In a recent publication: “LT175 is a novel ...
A novel insights on our repTOP ERE-Luc mouse model
In a recent publication: “Estrogen receptor...
Join TOP srl at the 12th FELASA and 12th SECAL joint congress entitled: ‘Animal Research: Better Science from Fewer Animals’ (10-13 June 2013 Barcelona)
This year at the FELASA meeting, TOP srl will...
Join TOP srl at the American Association for Cancer Research (AACR) Annual Meeting 2013 (April 6-10 2013 Washington DC)
This year at the AACR meeting, TOP srl will p...

Validation: the S.A.T.U.R.N. concept

Backed by an academic experience of more than a decade, TOP srl checks the usefulness and reliability of repTOP™ mice through a standard validation procedure.


'S' for SPECIFICITY

Reporter systems rely on an easy-to-detect marker gene under the control of a specific promoter.
SATURN validation bypasses risk factors which can compromise specificity


'A' for ACCURACY

repTOP™ mice are an accurate model:
- Quantitative signal readout
- Sensitive over a broad range of agonist concetrations, comprising physiological ones
- Ability to distinguish the pharmacological profile of different compounds, and to identify novel effects of selective receptor modulators (SRMs).


“T” for TURN-OVER

repTOP™ biomarkers, characterized by high turnover rate, provide a temporal dimension in the study of drug action.


'U' for UBIQUITY

repTOP™ technology, thanks to the use of insulators, allows us to overcome positional effects that hamper ubiquitous and tightly regulated transgene expression. The SATURN validation process guarantees the availability of the biomarker in all the tissues: wherever and whenever your agonist is present, you will get the signal!


‘R’ for REPRODUCIBILITY

repTOP™ mice are a reproducible model:

           -  Low inter-individual variability achieved by control of chromosomal positional effects

-           - Easy comparison of experiments performed at different times/age and different drugs.


'N' for NON INVASIVE

Imaging offers highly informative in vivo studies while reducing the use and minimising animal suffering and stress. The non-invasive nature of the model enables the investigation of physiological events from embryos to elderly animals, as well as very precise longitudinal studies of drug action. Our commitment to improving human health by accelerating the discovery of innovative therapies is always coupled with respect for ethical standards in research.


Seeing research in a better light

TOP strives for an ethical use of animal models in full compliance with the 3Rs rule. With its alternative models, TOP intends to nullify animal suffering, * Reduce animal use, * Replace current in vivo methodologies and * Refine biomedical research tools with the generation of more predictive models for the study of the molecular basis of human diseases and the effects of therapies.